Coding

Part:BBa_K1394002:Design

Designed by: Sean Lowe   Group: iGEM14_Melbourne   (2014-10-05)


MAP star peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein was designed to both be highly soluble and lacking sequences which would make it prone to proteolytic degradation.


Source

The peptide is completely synthetic and was designed by the University of Melbourne iGEM team. It is expressed with the fusion partner, SUMO, to improve solubility. The SUMO protein sequence was derived from the work of TU Delft 2013 iGEM. The DNA sequence for this protein was codon optimised for E. coli.

References